Update on the Diagnosis and Treatment of Mycoplasma Haemofelis and M. Haemominutum Infections of Cats

ONE Parenteral administration of feline viral rhinotracheitis, calicivirus, and panleukopenia vaccines induces antibodies that react with Crandall Reese feline kidney cell lysates and feline renal cell lysates. Michael R. Lappin, DVM, PhD, Wayne A. Jensen, DVM, PhD, Tracey D. Jensen, DVM, MS, et al. In review, Am J Vet Res 2004. The viruses used in many commercially available feline herpesvirus 1, calicivirus, and panleukopenia (FVRCP) vaccines are grown on the Crandall Reese Feline Kidney (CRFK) cell line. We vaccinated two kittens with each of four commercially available FVRCP vaccines grown on the CRFK cell line. The kittens were vaccinated three times as kittens and boosted once on week 50 of the study. Two additional kittens per group each were inoculated with 10 μg, 50 μg, or 50 μg CRFK lysate plus alum 11 times over the 50 week study period. Renal biopsies for histopathological evaluation were collected prior to the study and on week 56. CBC, serum biochemical analysis, urinalysis, microalbuminuria assay, ELISA for detection of antibodies against CRFK lysate, and ELISA for detection of antibodies against lysed feline renal cells (FRC) were performed on samples collected throughout the study. Antibodies against CRFK antigens were detected by ELISA in each cat inoculated with CRFK lysate and five of six cats administered a parenteral FVRCP vaccine. Antibodies against FRC were detected by ELISA in all the cats administered CRFK lysate alone or a parenteral FVRCP vaccine. Clinical pathological and histopathological abnormalities were minimal. Parenteral administration of vaccines containing CRFK proteins induces CRFK antibodies and FRC antibodies in some cats. However, the clinical pathological and histopathological results suggest that hypersensitization with CRFK proteins is not associated with detectable renal disease in the time period studied.